維生素-水溶性（梯度iii）vitamins – water soluble (gradient iii)
conditions
column: ace 3 c18-ar
dimensions: 150 x 4.6 mm
part number: ace-119-1546
mobile phase: a: 20 mm potassium phosphate ph 2.83 in h2o
b: 20 mm potassium phosphate ph 2.83 in meohh2o (50:50 v/v)
gradient:
time(mins)
%b
0
20
15
70
flow rate: 1.5 ml/min
injection: 1 μl
temperature: 40 °c
detection: uv, 205 nm
analytes
1. pyridoxine (vitamin b6)
2. pantothenic acid (vitamin b5)
3. p-aminobenzoic acid
4. folic acid (vitamin b9/ vitamin m)
5. d-biotin (vitamin b7/ vitamin h)
6. cyanocobalamin (vitamin b12)
7. riboflavin (vitamin b2)